New insight into selecting appropriate primers and databases when sequencing soil nematode DNA

Soil nematodes represent a major component of soil communities in terrestrial ecosystems, and play an important role in regulating nutrient cycling and soil health. High-throughput sequencing technology is increasingly used in the study of nematode biodiversity. A recent study demonstrates that the selection of different primers and databases influenced the annotation of nematode taxa, but the diversity of the nematode community showed consistent patterns among different vegetation types.

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